Standard solution preparation: Weigh accurately about 100mg o-Xylene in a 100ml volumetric flaskadd acetonitrile and mix well and make up the volume up to mark. Label as stock solution of Xylene.Weigh accurately about 40mg of isothiocyanate standard in 25ml volumetric flask, transfer 2mlXylene stock solution with the help of a pipette, add about 20ml acetonitrile and sonicate todissolve.Allow the contents to cool to room temperature and make up the volume with diluent. Note down theweight. Prepare the standard solution in duplicate